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Molecular Microbiology

Molecular biology is the study of biology at a molecular level. The field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between DNA, RNA and protein biosynthesis, the transcription factor as well as learning how these interactions are regulated.

It is obvious why open access and open data portals would be beneficial to the public: free information and more of it is empowering to society. Molecular Biology Methods are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid.

The methods of molecular biology includes:

1)Hemacytometer for cell count, 2)Restriction enzyme digest(a process of cutting DNA molecules into smaller pieces with special enzymes called restriction endonucleases), 3)DNA ligation( using DNA ligase enzyme that helps in joining the DNA strands together by catalysing the formation of a phosphodiester bond), 4)Transfection( a process of introducing nucleic acids into the cells and used mainly for non-viral methods in eukaryotic cells), 5)Western Blot(also called protein immunoblot used as an analytical thechnique to detect specific proteins ina sample of tissue homogenate or extract and uses gel electrophoresis in separation of native proteins by 3-D structure or denatured proteins by length of the polypeptide), 6)Plasmid Purification(mainly used to isolate plasmid DNA from bacterial cells using various reagents like isopropanol chloroform etc., 7)ELISA(a test that uses antibodies and color change to identify a substance), 8)Electroporation, 9)Heat shock method, SDS-PAGE, DNAgel Electrophoresis, PCR, Passaging cells(which mainly expand the number of cells or microorganisms in the culture) also called subculturing and molecular cloning.